Table 2. Types of spontaneous CAN1 mutations in pol2-4,eex msh6Δ strains.
|
pol2-4,eex msh6Δ |
||||||||
|---|---|---|---|---|---|---|---|---|
| WTa | msh6Δa | pol2-4 msh6Δ | G435C | V522A | T850Mb | K966Qb | A1153D | |
| Transitions | ||||||||
| G→A | 8 (24) | 16 (34) | 20 (36) | 19 (41) | 10 (19) | 21 (41) | 26 (48) | 21 (41) |
| C→T | 3 (9) | 2 (4) | 5 (9) | 4 (9) | 8 (15) | 4 (8) | 6 (11) | 4 (8) |
| A→G | 0 (0) | 0 (0) | 0 (0) | 0 (0) | 0 (0) | 1 (2) | 0 (0) | 0 (0) |
| T→C | 0 (0) | 3 (6) | 0 (0) | 0 (0) | 0 (0) | 1 (2) | 4 (7)c | 1 (2) |
| Total | 11 (33) | 21 (45) | 25 (45) | 23 (50) | 18 (35) | 27 (51) | 36 (67)c | 26 (51) |
| Transversions | ||||||||
| G→T | 2 (6) | 5 (11) | 21 (38) | 23 (50) | 29 (56) | 6 (12)c | 12 (22) | 19 (37) |
| C→A | 3 (9) | 10 (21) | 1 (2) | 0 (0) | 0 (0) | 8 (16)c | 4 (7) | 1 (2) |
| G→C | 1 (3) | 0 (0) | 0 (0) | 0 (0) | 0 (0) | 0 (0) | 0 (0) | 1 (2) |
| C→G | 2 (6) | 0 (0) | 0 (0) | 0 (0) | 0 (0) | 0 (0) | 0 (0) | 0 (0) |
| A→C | 1 (3) | 1 (2) | 0 (0) | 0 (0) | 0 (0) | 0 (0) | 0 (0) | 0 (0) |
| T→G | 1 (3) | 2 (4) | 0 (0) | 0 (0) | 0 (0) | 1 (2) | 0 (0) | 0 (0) |
| A→T | 1 (3) | 0 (0) | 0 (0) | 0 (0) | 0 (0) | 1 (2) | 0 (0) | 0 (0) |
| T→A | 1 (3) | 0 (0) | 0 (0) | 0 (0) | 0 (0) | 1 (2) | 0 (0) | 0 (0) |
| Total | 12 (36) | 18 (38) | 22 (39) | 23 (50) | 29 (56) | 17 (33) | 16 (30) | 21 (41) |
| Frameshifts | ||||||||
| +1 | 2 (6) | 3 (6) | 9 (16) | 0 (0)c | 3 (6) | 3 (6) | 2 (4)c | 3 (6) |
| −1 | 2 (6) | 1 (2) | 0 (0) | 0 (0) | 2 (4) | 5 (10)c | 0 (0) | 1 (2) |
| 4 (12) | 4 (9) | 9 (16) | 0 (0)c | 5 (10) | 8 (16) | 2 (4)c | 4 (8) | |
| Other | 6 (18) | 4 (9) | 0 (0) | 0 (0) | 0 (0) | 0 (0) | 0 (0) | 0 (0) |
| Multiple | 0 (0) | 0 (0) | 1 (2) | 1 (2) | 1 (2) | 0 (0) | 1 (2) | 0 (0) |
| Total | 33 | 47 | 56 | 46 | 52 | 51 | 54 | 51 |
The CAN1 genes were sequenced from independent Canr mutants of each strain. The numbers of mutations of each subtype are shown with percentages in parentheses. Transitions and transversions indicate base changes observed in the coding strand of CAN1 (as in Figure 6). “Other” includes duplications, deletions, and complex mutations. Some mutants had two mutations separated by >10 bp (61–1150 bp); these are reported under ‘”Multiple”; each mutation in this category was scored as an independent event and added to the relevant subclass tally, although they may be mechanistically linked. See Figure 6 for locations of mutations in the CAN1 gene.
WT and msh6Δ data from Herr et al. (2011a).
Distribution of base substitutions significantly different from pol2-4 msh6Δ (P ≤ 0.05, Monte Carlo hypergeometric test).
Significantly different from pol2-4 msh6Δ (P ≤ 0.05, Fisher’s exact test).