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. 2013 Mar;193(3):819–828. doi: 10.1534/genetics.112.148635

Figure 2.

Figure 2

X overexpression in F1 hybrid male testes is associated with a musculusPWK introgression on the proximal X. (A) Sex chromosome genotypes and mean X overexpression in experimental males. X introgression genotypes are numbered 1–7; regions of uncertainty between domesticusLEWES (open) and musculusPWK (solid) recombination break points on the X are shown shaded; all genoytpes share the same F1 autosomal background. Expression was normalized relative to beta-actin, Acrv1, and Protamine 1CT); mean ΔΔCT is the difference in expression between experimental genotypes and the fertile F1, averaged across the 12 X-linked genes indicated on the left side. Error bars are +1 SE. Tests for significant differences between experimental genotypes and control were performed on ΔCT values with ANOVA followed by Dunnett post hoc tests, (*) P < 0.01, (***) P < 0.0001. (B) Overexpression of individual X-linked genes does not require a musculusPWK allele. Data are presented as genotype means (±1 SE) for six representative genes. Sex chromosome genotypes are shown on the x-axis. Solid bars denote the musculusPWK allele at a given locus; open bars denote the domesticusLEWES allele. Significance was tested as in 2A, (*) P ≤ 0.05, (**) P ≤ 0.001. See also Figure S2.