Figure 5.

Effect of the GPN-IWR1 system on RNA polymerase III localization. (A) Nuclear localization of Rpc53-GFP in gpn2-4 and gpn3-1 mutants. (B) Schematic of nuclear localization scoring system and quantification of Rpc53-GFP localization from A. α indicates the results of Tukey’s posthoc analysis of a one-way ANOVA for the three data sets. (C) Spot dilution assays of GPN2 (left) and GPN3 (right) mutants in combination with RNAPI and III subunit mutant alleles. (D) Localization of Rpc37–GFP and Rpc40–GFP in iwr1Δ. (E) Quantification of Rpc37–Rpc40– and Rpo31–GFP fusions using the scoring system from B. P-values indicate the results of Student’s t-test.