Table 1. Percentage developmental progression of cloned and parthenogenetic embryos prepared with eggs from different strains.
|
BXD Strain | ||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| SCNT | 1 | 2 | 5 | 6 | 8 | 9 | 11 | 12 | 13 | 14 | 15 | 16 | 18 | 19 | 21 | 22 |
| Two cell/construct | 59.6 | 75.4 | 77.4 | 38.2 | 80.7 | 97.7 | 79.8 | 11.6 | 57.1 | 71.2 | 80.6 | 82 | 66 | 84.4 | 85.2 | 77.4 |
| Four cell/two cell | 0 | 20.5 | 52.3 | 1.2 | 28.1 | 15 | 64.2 | 15.3 | 53.9 | 17.4 | 4 | 15.2 | 42.1 | 49.7 | 14.7 | 54.5 |
| Blastocyst/two cell | 0 | 7.6 | 11.9 | 1.2 | 6.8 | 4.5 | 27.5 | 0 | 16.5 | 5.7 | 0 | 0 | 10.4 | 4.6 | 4.5 | 11 |
| Blastocyst/four cell | 0 | 29.5 | 17.9 | 25 | 16.8 | 31.9 | 43 | 0 | 34.5 | 27.3 | 0 | 0 | 25.4 | 9.1 | 31.3 | 19.3 |
| 27 | 28 | 29 | 31 | 32 | 33 | 34 | 36 | 38 | 39 | 40 | 42 | B6 | D2 | F1 | ||
| Two cell/construct | 51 | 82.1 | 78.5 | 43.8 | 70.1 | 15.6 | 84.8 | 86.3 | 56.9 | 72.4 | 93.5 | 23.1 | 68.3 | 64.7 | 87.3 | |
| Four cell/two cell | 59 | 29.8 | 10.7 | 7.3 | 50.6 | 0 | 48.9 | 37.2 | 45.8 | 3 | 66 | 39.2 | 53.6 | 43 | 66 | |
| Blastocyst/two cell | 23.7 | 8.7 | 0.9 | 5.2 | 8 | 0 | 13.9 | 17.5 | 4.3 | 1.2 | 16.1 | 7 | 20.9 | 0 | 27.4 | |
| Blastocyst/four cell | 37.9 | 32.9 | 5.8 | 55.6 | 21.5 | 0 | 26.6 | 54.4 | 6.9 | 27.8 | 24.2 | 10.7 | 37.9 | 0 | 36.3 | |
| Parthenote | 1 | 2 | 5 | 6 | 8 | 9 | 11 | 12 | 13 | 14 | 15 | 16 | 18 | 19 | 21 | 22 |
| Two cell/activated | 85.4 | 97.4 | 91.9 | 98.2 | 91.7 | 97.7 | 95.7 | 22.7 | 97.4 | 78.4 | 97.5 | 99.2 | 80.2 | 100 | 98.6 | 95.7 |
| Four cell/two cell | 5.3 | 100 | 59.3 | 83.1 | 52.9 | 86.4 | 95 | 79.5 | 77.8 | 55 | 49.2 | 9.4 | 73.6 | 81.4 | 79.3 | 78 |
| Blastocyst/two cell | 0 | 91.5 | 5.6 | 0 | 2.5 | 38.8 | 83.6 | 7.3 | 38.7 | 11.7 | 33.7 | 0 | 40.9 | 18.8 | 57.5 | 12.6 |
| Blastocyst/four cell | 0 | 91.5 | 7.9 | 6.1 | 3.6 | 44.6 | 88.8 | 7.5 | 46.9 | 16.7 | 62.8 | 0 | 54.8 | 23.4 | 72 | 16.4 |
| 27 | 28 | 29 | 31 | 32 | 33 | 34 | 36 | 38 | 39 | 40 | 42 | B6 | D2 | F1 | ||
| Two cell/construct | 66.7 | 97.5 | 91.3 | 95.1 | 94.3 | 19.4 | 100 | 90.1 | 66.3 | 84.4 | 95.7 | 39.6 | 84 | 72.6 | 100 | |
| Four cell/two cell | 100 | 97.1 | 31.7 | 85.2 | 61.3 | 46.7 | 89.8 | 60.1 | 13.5 | 84.6 | 100 | 80 | 100 | 59 | 99.6 | |
| Blastocyst/two cell | 77.1 | 64.7 | 3.3 | 39.9 | 22.5 | 12.5 | 48.6 | 14.7 | 3.8 | 61.5 | 74.5 | 8.3 | 36.8 | 2.1 | 72.2 | |
| Blastocyst/four cell | 77.1 | 66.4 | 6.7 | 46.7 | 39.5 | 17 | 54.7 | 26.3 | 35 | 73.8 | 74.5 | 8.3 | 36.8 | 2.5 | 72.6 | |
| SCNT:parthenote | 1 | 2 | 5 | 6 | 8 | 9 | 11 | 12 | 13 | 14 | 15 | 16 | 18 | 19 | 21 | 22 |
| Two cell/construct | 69.8 | 77.5 | 84.2 | 38.9 | 88 | 100 | 83.4 | 50.9 | 58.7 | 90.8 | 82.6 | 82.7 | 82.3 | 84.4 | 86.4 | 80.9 |
| Four cell/two cell | 0 | 20.5 | 88.2 | 1.4 | 53.2 | 17.4 | 67.5 | 19.2 | 69.3 | 31.7 | 8 | 163 | 57.2 | 61.1 | 18.6 | 69.9 |
| Blastocyst/two cell | 0 | 8.3 | 215 | 0 | 273 | 11.5 | 32.9 | 0 | 42.7 | 48.9 | 0 | 0 | 25.4 | 24.7 | 7.9 | 87.7 |
| Blastocyst/four cell | 0 | 32.3 | 227 | 408 | 469 | 71.4 | 48.4 | 0 | 73.5 | 164 | 0 | 0 | 46.2 | 39.1 | 43.4 | 118 |
| 27 | 28 | 29 | 31 | 32 | 33 | 34 | 36 | 38 | 39 | 40 | 42 | B6 | D2 | F1 | ||
| Two cell/construct | 76.5 | 84.2 | 86.1 | 46.1 | 74.3 | 80.3 | 84.8 | 95.7 | 85.8 | 85.9 | 97.7 | 58.4 | 81.3 | 89 | 87.3 | |
| Four cell/two cell | 59 | 30.7 | 33.7 | 8.6 | 82.5 | 0 | 54.4 | 61.9 | 339 | 3.6 | 66 | 49.1 | 53.6 | 72.9 | 66.3 | |
| Blastocyst/two cell | 30.7 | 13.4 | 28.9 | 13 | 35.7 | 0 | 28.6 | 119 | 115 | 2 | 21.6 | 84.5 | 56.8 | 0 | 37.9 | |
| Blastocyst/four cell | 49.2 | 49.6 | 87.7 | 119 | 54.5 | 0 | 48.7 | 207 | 19.8 | 37.7 | 32.5 | 129 | 103 | 0 | 49.9 | |
Values show the percentage progression from SCNT construct to two-cell stage, from two-cell to four-cell stage, from two-cell stage to blastocyst stage, and from four-cell stage to blastocyst. Development was scored for SCNT embryos and for parthenotes made with oocytes from the 28 strains. The ratio of SCNT:parthenote development was also calculated for each strain.