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Formation and quantification of co-integrate alleles in evolving populations. (A) Co-integrates were formed through the incomplete transposition (or subsequent recombination) of ISMex25 from the endogenous p2META plasmid into a small region of the trfA replication gene of the introduced pCM410 plasmid. (B) PCR amplification with pairs of primers internal to ISMex25 and flanking trfA allowed multiple, coexisting trfA::ISMex25 alleles to be quantified.
