Table 1. Mass Spectrometry Identification of PRAME Binding Proteins.
Band | Proteins identified | Number of peptides | Mowse score |
1 | ELB | 6 | 359 |
Histone H2B | 2 | 73 | |
2 | ELC | 6 | 397 |
Histone H4 | 2 | 108 |
GST-PRAME binding proteins were affinity purified as described in Materials & Methods. Bands of approximately 17 kDa and 12 kDa (Fig. 3A) were excised for mass spectrometry analysis. MS/MS fragmentation data were used to search the human NCBI database using the MASCOT search engine. Probability-based Mowse scores are shown.