Skip to main content
. 2013 Feb 27;8(2):e57796. doi: 10.1371/journal.pone.0057796

Figure 2. Regulation of RBP4 production by selected cytokines from MacCM.

Figure 2

SGBS adipocytes were treated with TNF-α, IL-1β, IL-6 or IL-8 for 48 hours. (A) mRNA expression of RBP4 was measured by quantitative PCR. RBP4 mRNA expression was normalized to succinate dehydrogenase complex subunit A (SDHA) and related to medium control using 2−ΔΔCT method. Data are presented as mean+SEM of three independent experiments. *p<0.05 (treatment vs. vehicle). (B) Accumulation of RBP4 in the medium supernatant was measured by ELISA. Measurements were related to untreated medium control. Data are presented as mean+SEM of three independent experiments. *p<0.05 (treatment vs. vehicle).