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. 2013 Feb 27;8(2):e57794. doi: 10.1371/journal.pone.0057794

Table 2. Primers designed and used in this study.

Primer name Sequence 5′–3′ Position (bp) Source
iutA-F CGAAGCTTTCTCAACCCACTGCTTCTT (iutA sense; Hind III site underlined) 33–51 This study
iutA-R TAGGATCCTGGTATAGCCATCGACCTT (iutA antisense; BamH I site underlined) 1981–1999 This study
iucA-F CTCAAGCTTAGTGCTTCCTGAATGCCT (iucA sense; Hind III site underlined) 44–61 This study
iucA-R CTCGGATCCTATGAGTCACCTGGTCAC (iucA antisense; BamH I site underlined) 1556–1573 This study
pS-iucA-F CTCGATATCTCAGGCAGGTATCGTTCA (pS-iucA sense; EcoR V site underlined) 492–509 This study
pS-iucA-R CTCGAATTCCGATGCTTACTGTCAGCA (pS-iucA antisense; EcoR I site underlined) 1200–1217 This study
iucC-F CTCGAATTCACTGGGATTTGGTCAACC (iucC sense; EcoR I site underlined) 14–31 This study
iucC-R CTCTCTAGAATTCCTGAGTTACCAGCC (iucC antisense; Xba I site underlined) 1715–1732 This study
Zeo-BamH I-F CTCGGATCCCACGTGTTGACAATTAAT (Inserting zeocin gene; BamH I site underlined) 1938–1955 This study
Zeo-BamH I-R CTCGGATCCTCAGTCCTGCTCCTCGGC (Inserting zeocin gene; BamH I site underlined) 2366–2383 This study
GF CACAGTCTTACTGCCAGT (shiG sense) 114–131 This study
GR TTCTCGGTATCGGACAGA (shiG antisense) 266–283 This study
BF TTGGTGAACAGCAATGGC (iucB sense) 701–718 This study
BR ACCTCCGTGAAGAAGTGA (iucB antisense) 921–938 This study
DF ATGGCATCACTGCCGATT (iucD sense) 782–799 This study
DR TACGTGCAGATCTCCATG (iucD antisense) 1181–1198 This study
O2F ATGTCGTGTTCCGTGCTCA (O2ColV sense) 1–19 This study
O2R TCAGTAAGTTGGCAGCATC (O2ColV antisense) 204–222 This study
Re-iucA-F TCAGGATCCATGATCCTGCCCTCTGAA (iucA sense; BamH I site underlined) 1–18 This study
Re-iucA-R TCAGAATTCTCAGACCTCCTGAGCCTG (iucA antisense; EcoR I site underlined) 1708–1725 This study
Re-iucC-F CGCGAATTCATGAATCACAAAGACTGG (iucC sense; EcoR I site underlined) 1–18 This study
Re-iucC-R GCGGTCGACTCATGATTCATATTCCTG (iucC antisense; Sal I site underlined) 1726–1743 This study