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. 2013 Feb 27;8(2):e58056. doi: 10.1371/journal.pone.0058056

Figure 3. Several motifs of K3 and K5 are required for DC-SIGN modulation.

Figure 3

293 stable cell lines expressing DC-SIGN (panels A and C) or DC-SIGNR (panels B and D) were transiently transfected with 4 µg of GFP expression construct for wild-type or the indicated mutants of K3 (panels A and B), K5 (panels C and D) or GFP control vector. At 36-48 hours post-transfection, the cells were harvested and surface stained for DC-SIGN, DC-SIGNR, and MHC class I. Using flow cytometry, live cells were gated for GFP expression and the geometic mean channel florescence was normalized to GFP vector expressing cells. The data are an average of three independent experiments and error bars indicate standard deviations.