Figure 2. Acute BDNF treatment increases GluR1-SAP97 and GluR2-GRIP1 interactions in neurons and enhances GluR1 and GluR2 expression on neuronal cell surface.

DIV 14 cultured neurons acutely-treated with BDNF (1 h, 50 ng/ml), or not, are lysed and processed for co-immunoprecipitation using anti-SAP97 (A) and anti-GRIP1 (B). Immunoblots are probed with anti-GluR1 and anti-GluR2 (upper blots in A and B, respectively). Re-probing the same blots with anti-SAP97 and anti-GRIP1 indicates equal loading (lower blots in A and B, respectively). C) Acute BDNF treatment (1 h, 50 ng/ml) does not increase total GluR1 and GluR2 protein levels in similarly treated cultures. D) Similarly treated cultures, as in (C), are treated with biotinylation reagent and biotinylated total surface proteins are analyzed with anti-GluR1 and anti-GluR2 antibodies. Results are representative of 4 experiments; *: p<0.01; Student’s t-test.