Figure 6. BMMC show mediator release in response to bacterial PAMPs and L. monocytogenes in an LLO-dependent manner.

(A, B, C, D and F) BMMC were incubated with media alone, WT or Δhly L. monocytogenes at the MOI indicated for 2 h, washed and transferred to media containing 50 µg/ml gentamicin for an additional 24 h. Cell supernatants were harvested after the initial 2 h (open bars) and the additional 24 h (grey bars). (E) BMMC were incubated with media alone, 100 ng/ml LPS or 10 µg/ml PGN for 24 h. (B) IL-6, (C) CCL2, (D) TNF-α, and (E and F) OPN concentrations were determined by ELISA, with values below the limit of detection indicated (ND). Data are mean +/- SEM for (B) 5, (C) 4, (D) 4, (E) 8, and (F) 3 independent experiments. Statistical significance p<0.05 compared to media treatment is indicated (*).