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. 2013 Feb 27;8(2):e57102. doi: 10.1371/journal.pone.0057102

Figure 7. IL-6 and CCL2 secretion in response to L. monocytogenes is MyD88-dependent.

Figure 7

BMMC from MyD88+/- and MyD88-/- mice were incubated with media alone, WT L. monocytogenes at the MOI indicated, 100 ng/ml LPS or 100 µg/ml PGN for 2 h, washed and transferred to media containing 50 µg/ml gentamicin for an additional 24 h. Cell supernatants were harvested at the times indicated for MyD88+/- (open bars) and MyD88-/- (grey bars), and (A) IL-6 and (B and C) CCL2 levels were determined by ELISA. Data are mean +/- SEM for (A) 6; (B and C) 5 independent experiments. Statistical significance p<0.05 compared to media treatment is indicated (*).