Skip to main content
. 2013 Feb 27;8(2):e57307. doi: 10.1371/journal.pone.0057307

Table 1. Antibodies/Lectin used for histology.

Antibody/Lectin (source) Specificity Dilution
Paraffin Cryostat
Polyclonal rabbit anti-Iba1 reactive with human, mouse and rat Iba1 (Wako Chemicals, Neuss, Germany) Microglia/ macrophages 1/500
Monoclonal rat anti- mouse CD68 (Serotec, Düsseldorf, Germany) Microglia/ macrophages 1/200
Polyclonal rabbit anti-Laminin reactive with human and mouse Laminin (Sigma-Aldrich, Munich, Germany) Basal lamina 1/50
Polyclonal rabbit anti -human GFAP (Dako, Hamburg, Germany) Glial fibrillary acidic protein 1/200
Biotin-conjugated tomato lectin, L. esculentum (Axxora, Lörrach, Germany ) Microglia/ macrophages, endothelial cells 1/50
Monoclonal mouse anti-mouse NeuN (Chemicon, Schwalbach, Germany) Neurons 1/200
Monoclonal mouse anti-mouse PLP (Serotec, Düsseldorf, Germany) Proteo-Lipid-protein 1/500