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. 2013 Feb 27;8(2):e57074. doi: 10.1371/journal.pone.0057074

Figure 2. Reconstitution of TGFBR2 expression.

Figure 2

(A) Real-time RT-PCR analysis of endogenous mutant (A9), transgenic wildtype (A10) or both TGFBR2 transcripts in the absence and presence of dox (1 µg/ml) are shown. Results represent the mean of three independent observations ±S.D. (B) Western blot analysis, demonstrating the presence of dox-inducible (1 µg/ml) TGFBR2 expression in a time-dependent manner. ß-Actin served as a loading control. Data are shown for HCT116-TGFBR2 clone #5, but also apply to clone #22 (data not shown).