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. 2013 Feb 27;8(2):e57074. doi: 10.1371/journal.pone.0057074

Figure 3. Reconstitution of TGFBR2 signaling.

Figure 3

(A) Phosphorylation of SMAD2 (pSMAD2) was detected by Western blot analysis. Treatment with dox (1 µg/ml) and TGF-ß1 (10 ng/ml) displayed higher levels of pSMAD2 in comparison to cells grown in the absence of dox. The parental HCT116 cell line served as negative control, whereas TGF-ß1 responsive HepG2 cells were used as a positive control. Total SMAD2 has been used as a loading control. (B) Target gene transcription of TGFBR2 signaling. Real-time RT-PCR experiments revealed dox-dependent SMAD7 and SERPINE upregulation. Data are shown for HCT116-TGFBR2 clone #5 but also apply to clone #22 (data not shown). Values represent the means of three independent experiments ±S.D.