Figure 1. Immunoblotting showed the coupling of Aβ1-42 to USPIO using anti-Aβ monoclonal antibody 6E10.

Lane 1 shows USPIO-PEG-Aβ1-42 in which Aβ1-42 coupled to nanoparticles is mainly in the stacking gel. A small proportion of Aβ1-42 which was not covalently bound to the particles entered the running gel. Lane 2 shows USPIO alone as negative control, with no bands seen. Lane 3 shows synthetic Aβ1-42 as a positive control, where the two bands seen are monomeric and dimeric Aβ1-42.