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. Author manuscript; available in PMC: 2013 May 1.
Published in final edited form as: J Nutr Biochem. 2012 Jul 26;24(5):770–775. doi: 10.1016/j.jnutbio.2012.04.007

Fig. 3.

Fig. 3

Effects of PFKFB3/iPFK2 disruption on dietary response of intestine iPFK2. (A) Validation of heterozygous PFKFB3/iPFK2 disruption. Genomic DNA of PFKFB3+/− mice and wild-type littermates was subjected to PCR analyses using an exon 2-specific primer with an exon 3-specific primer (WT) or a neomycin-specific primer (Neo). (B) Male PFKFB3+/− mice and wild-type littermates, at 5–6 weeks of age, were fed an LFD or HFD for 12 weeks. Intestine lysates were prepared to determine the amount of iPFK2 using Western blot analyses.