Skip to main content
. Author manuscript; available in PMC: 2013 May 1.
Published in final edited form as: J Nutr Biochem. 2012 Jul 26;24(5):770–775. doi: 10.1016/j.jnutbio.2012.04.007

Fig. 5.

Fig. 5

Alterations of intestine microbiota composition. Male PFKFB3+/− mice and wild-type littermates, at 5–6 weeks of age, were fed an HFD for 12 weeks and treated with rosiglitazone (Rosi, 10 mg/kg/day in PBS) or vehicle (PBS) orally for the last 4 weeks of the feeding regimen. Before, during and after treatment with rosiglitazone or PBS, fecal samples of HFD-fed mice were collected, pooled and homogenized. Total genomic DNA was isolated and subjected to quantitative real-time PCR using primers specific to Lactobacillus (A) and Bifidobacterium (B). Ct, cycle threshold.