Table 1.
Primers, strains, and plasmids
| Primer/strain/plasmid | Genotype/description | Source/reference |
|---|---|---|
| Primers | ||
| pilD-5′ | 5′-GGTTGCGATCGCCATGCCCCTCCTCGACTACCTG-3′ | This study |
| pilD-3′ | 5′-GTGTGTTTAAACTTTGAATCCGGCGAATTGCAGATAGG-3′ | This study |
| pilA-5′ | 5′-GGTTGCGATCGCCATGAAAGCTCAAAAAGGCTTTACCT-3′ | This study |
| pilA-3′ | 5′-GTGTGTTTAAACTCAGTTATCACAACCTTTCGGAGTGAACAT-3′ | This study |
| flaK-5′ | 5′-GGTTGCGATCGCCATGGCATATACAATAGGACTTTTGGGA-3′ | This study |
| flaK-3′ | 5′-GTGTGTTTAAACCAATGGCAATATAATACTAATTATTTTA-3′ | This study |
| flaB2-5′ | 5′-GGTTGCGATCGCCATGAAAATAAAAGAATTCATGAGTAACAAAAA-3′ | This study |
| flaB2-3′ | 5′-GTGTGTTTAAACCTATTGTAATTGAACTACTTTTGAATCGTTTAA-3′ | This study |
| Strains | ||
| Escherichia coli JM109 | endA1 gyr96 thi hsdR71 supE44 recA1 relA1 Δ(lac-proAB) (F' traD36 proAB+ lacIq lacZΔM15) | Yanisch-Perron et al. (1985) |
| Plasmids | ||
| pEU-C-His Flexi | Ampr, SP6 promoter expression vector | Sevova et al. (2010) |
| pFlexipilD | pEU-C-His Flexi 870 bp pilD gene from Pseudomona aeruginosa strain PAK | This study |
| pFlexipilA | pEU-C-His Flexi 450 bp pilA gene from P. aeruginosa strain PAK | This study |
| pFlexiflaK | pEU-C-His Flexi 699 bp flaK gene from Methanococcus voltae | This study |
| pFlexiflaB2 | pEU-C-His Flexi 651 bp flaB2 gene from M. voltae | This study |
Sgf1 restriction enzyme cleavage sites in 5′ (forward) primers and PmeI sites in 3′ (reverse) primers are underlined, native start and stop codons are italicized.