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. 2013 Jan 4;2(1):161–172. doi: 10.1002/mbo3.54

Figure 1.

Figure 1

Mutation in the carbon metabolism regulator, Crc, reduced the expression of exsCEBA. (A) Transcriptional expression of PexsC–lacZ in crc transposon mutant (p39) and deletion mutant (Δcrc) as well as their corresponding crc-complemented strains under type III secretion system-inducing condition. The parental strain PAO1pClacZ (pcZ), which carried a single copy of PexsC–lacZ transcriptional reporter gene on its chromosome and its exsA transposon mutant (exsA::Tn) were used as positive and negative controls, respectively. (B) Immunoblotting detection of exsCEBA promoter-directed β-galactosidase. Total proteins were prepared from strain PAO1pClacZ (pcZ) and its derivatives. The expression levels were assayed by immunoblotting using antibodies against β-galactosidase. (C) Expression of PexsC–lacZ in crc deletion mutant under different growth conditions as indicated. Bacterial cultures were grown in Luria–Bertani (LB) medium, LB with 5.0 mmol/L nitrilotiracetic acid (LBnta), or Mins minimal medium. The data were the means of three replicates and error bar represents standard deviations.