Table 1.
Bacterial strains and plasmids used in this study1
| Strain or plasmid | Relevant genotype or phenotype | Source or reference |
|---|---|---|
| Pseudomonas aeruginosa | ||
| PAO1 | Prototrophic laboratory strain | Laboratory collection |
| PAO1pClacZ | PAO1 with chromosome integration of exsCEBA promoter fused with lacZ | Zhou et al. (2007) |
| p39 | Transposon mutant with disruption of crc gene in PAO1pClacZ | This study |
| P39(crc) | P39 containing pUCP-crc | This study |
| Δcrc | PAO1 with in-frame deletion of crc | This study |
| Δcrc(crc) | Δcrc containing pUCP-crc | This study |
| ΔcrcpClacZ | PAO1pClacZ with in-frame deletion of crc | This study |
| ΔcrcpClacZ(crc) | ΔcrcpClacZ containing pUCP-crc | This study |
| ΔcbrBpClacZ | PAO1pClacZ with in-frame deletion of cbrB | This study |
| ΔCrcZpClacZ | PAO1pClacZ with deletion of CrcZ | This study |
| exsA::Tn | Transposon mutant with disruption of exsA gene in PAO1pClacZ | This study |
| PAO1pTlacZ | PAO1 with chromosome integration of exoT promoter fused with lacZ | Zhou et al. (2007) |
| PAO1pDlacZ | PAO1 containing a vector of pME-PexsD–lacZ | This study |
| PAO1pD’-‘lacZ | PAO1 containing a vector of pME-PexsD’-‘lacZ | This study |
| PAO1pN’-‘lacZ | PAO1 containing a vector of pME-PpopN’-‘lacZ | This study |
| Escherichia coli | ||
| DH5α | F−ϕ80dlacZΔM15 endA1hsdR17 (rk− mk−. supE44 thi-1 gyrA96 Δ(lacZYA-argF) | Gibco |
| S17-1 | recA pro (RP4-2Tet::Mu Kan::Tn7) | Simon et al. (1983) |
| Plasmid | ||
| pBT20 | Mariner transposon vector; Gmr, Apr | Kulasekara et al. (2005) |
| pUCP19 | E. coli – P. aeruginosa shuttle vector with the lac promoter (Plac), Ampr/Cbr | ATCC 87110 |
| pUCP-crc | pUCP19 containing crc under the control of Plac | This study |
| pUCP-exsA | pUCP19 containing exsA under the control of Plac | This study |
| pEX18Gm | P. aeruginosa gene replacement vector; sacB, Gmr/Cbr | Hoang et al. (1998) |
| pEX-de crc | pEX18Gm carrying the crc flanking region with the gene being deleted | This study |
| pEX-de cbrB | pEX18Gm carrying the cbrB flanking region with the gene being deleted | This study |
| pEX-de CrcZ | pEX18Gm carrying the CrcZ flanking region with the gene being deleted | This study |
| pME6010 | pVS1-p15A shuttle vectors | Heeb et al. (2000) |
| pME2-lacZ | pME6010 carrying a full-length lacZ | Dong et al. (2008) |
| pME-PexsDlacZ | pME2-lacZ carrying exsD promoter in front of lacZ | This study |
| pME-PexsD’-‘lacZ | PexsD’-‘lacZ translational fusion | This study |
| pME-PpopN’-‘lacZ | PpopN’-‘lacZ translational fusion | This study |
1
Ampr, ampicillin resistant; Cbr, carbenicillin resistant; Gmr, gentamicin resistant.