. Author manuscript; available in PMC: 2014 Jun 1.

Published in final edited form as: Cancer Metastasis Rev. 2013 Jun;32(0):109–122. doi: 10.1007/s10555-012-9409-1

Table 2.

Promoter elements used to develop mouse models of prostate cancer

Promoter/Cre Allele	Relevant mouse models

Rat Probasin (PB): Minimal PB Expression cassette carrying 426 basepairs of the rat probasin (PB) promoter and 28 base pairs of 5′-untranslated region (−426/+28)	PB-T/tag(TRAMP) TRAMP crossed with BCL2 transgene TRAMP crossed with EGR1 null mice PB-mAR PB-Akt
Large PB (LPB) A large (L) fragment of the PB promoter (from 11 500 to +28) that retains some enhancer elements to facilitate high gene expression	LPB-Tag (LADY) LARGE T ANTIGEN
ARR₂PB A composite probasin promoter with androgen and glucocorticoid, prostate-specific, and achieves high levels of transgene expression	ARR₂PB-myc ARR₂PB-FGFR1 (JOCK1) T121 ARR₂PB ^CreERt2
PB-Cre/PB-Cre4 PB-Cre4 Cre gene under the control of the ARR₂PB	PB-^Cre4; Pten^flox/flox PB-^Cre4; Pten^flox/flox; p53^flox/flox PB-^Cre4; Pten^flox/flox; SMAD4^flox/flox PB-^Cre4; Apc ^flox/flox

Prostate Specific Antigen (PSA) PSA-CreERT2 6-kb fragment of the human PSA promoter driving expression of the nuclear-targeted modified Cre (tamoxifen-inducible)	PSACreERt2; Ptenflox^/flox

Nkx3.1 Prostate-specific homeobox gene regulated by androgen Nkx3.1^CreERt2–modified Cre knocked into the Nkx3.1 gene to express in prostate; nuclear-targeted modified Cre (tamoxifen-inducible)	Nkx3.1^CreERt2; Ptenflox^/flox Nkx3.1^CreERt2; Pten^flox/flox; Braf^LSLflox/+ Nkx3.1^CreERt2; Pten^flox/flox; Kras^LSLflox/+

HoxB13 Prostate-specific homeobox gene not regulated by androgen Hoxb13-rtTA–tetracycline regulator driven by HoxB13 promoter	Hoxb13-rtTA/TetO-H2BGFP