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. Author manuscript; available in PMC: 2013 Feb 28.
Published in final edited form as: Am J Rhinol Allergy. 2011 Sep-Oct;25(5):307–312. doi: 10.2500/ajra.2011.25.3643

Figure 4. The effects of quercetin on ciliary beat frequency in wild type and CFTR knockout cultures.

Figure 4

Figure 4

(A) Time dependent effects of stimulated CBF following a single application of apical quercetin (10 μM) in wild type and CFTR knockout cultures. (B) Quercetin (10 μM) significantly increased CBF in wild type nasal airway epithelial cultures both with and without the sodium-channel blocker, amiloride (100 μM). Relative stimulation of CBF was slightly enhanced in the presence of amiloride (1.85+/−0.18 vs. 1.44+/−0.17, p<0.01), although not significantly different when compared to cultures without sodium-channel blockade. Cells blocked with the CFTR inhibitor CFTRinh-172 completely suppressed quercetin-stimulated CBF. In addition, quercetin did not activate CBF in cultures derived from CFTR knockout mice over that observed with PBS alone (p=0.23). Incubation with amiloride increased CBF when exposed to both PBS (1.75+/−0.08) and quercetin (1.93+/−0.11). All CBF rates were obtained at 15 minutes.