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Western blot analysis of BAX and Bcl-2 protein expression in sham and treatment groups. The frozen hippocampi were lysed and transferred to nitrocellulose paper, then incubated with anti-Bcl-2, anti-Bax, and secondary anti-mouse antibodies. Bands were detected by chromogenic substrate. Data analyzed by one-way analysis of variance (ANOVA) followed by Tukey’s test for multiple comparisons.
*; p<0.05, MDMA vs. sham group.
#; p<0.05, ginger plus MDMA group vs. MDMA group.
