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. 2013 Feb 28;3:35. doi: 10.3389/fonc.2013.00035

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Copyright © 2013 Yehezkel, Shaked, Sagie, Berkovitz, Shachar-Bener, Segev and Selig.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in other forums, provided the original authors and source are credited and subject to any copyright notices concerning any third-party graphics etc.

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Premature senescence is associated with short telomere length in ICF fibroblasts. (A) TRF analysis of pCor fibroblasts at different PDs (which appear above the lanes). Mean telomere length (MTL) as determined by MATELO software are indicated below the lanes. Size markers in kb are shown to the left of the blot. (B) TRF analysis of pG fibroblasts with and without expression of ectopic hTERT. The PD at which DNA was extracted from the cells and the MTL in kb as determined by MATELO software, appear below the blot. (C) Methylation analysis of subtelomeric repeats with NBL-1 probe in pG and pCor fibroblasts after immortalization with hTERT. DNA samples were digested with either HpaII (H) or MspI (M) restriction enzymes. Blots were hybridized with NBL-1 probe. Arrows point to hybridization bands that appear in HpaII-digested DNA due to hypomethylation of NBL-1 repeats. (D) Methylation analysis of subtelomeric repeats with Hutel probe in pG and pCor fibroblasts after immortalization with hTERT. DNA samples were digested with either SauAI (S) or MboI (Mb) restriction enzymes. Blots were hybridized with Hutel probe. Arrows point to hybridization bands that appear in SauAI-digested DNA due to hypomethylation of Hutel repeats.