Table 1.

Primers for bisulfite analysis.

Sub-telomere	Forward primer	Reverse primer	Annealing temperaturea	Fragment size	References
2p	GTATTGTAGGTGTATAGTTGTATAAG	TAAAATTCCACCTATCTCTATAC	55/58°C	128 bp	Ng et al., 2009
4p	TGGTGTAGATGTAGAGAAGA	CTAACTTTTCAAATTACTAAAATTC	52/55°C	204 bp	Ng et al., 2009
5p	TTGATTTTGATTATTTAGGGGT	AAACAAAATACTCCTCAACACA	55/58°C	222 bp	Yehezkel et al., 2011
7q	TTTTTAAGGTTTGTGTTGAGG	TCTACACAACCTTTTAAAATAC	53/56°C	176 bp	Yehezkel et al., 2011
10q	TAATTGGTTTTTGATTTTGATT	CAAAATTCTTCTCAAATCAAAC	54/57°C	229 bp	Yehezkel et al., 2011
15q	TTTAGAGGGGTTTTTGTTTTTT	ACAAAATTCTCCTCAAATCAAA	56/56°C	273 bp	previously unpublished
16p	GTTTTAATTGGTTTTTGATTTTG	CAAAATTCTACTCAAATCAAAC	53/56°C	232 bp	previously unpublished
Xq	TATTTTGGGTATCATGTGTG	GCCACTGACTGGCTTTGGGAC	54/57°C	253 bp	Yehezkel et al., 2011

^aPCR following bisulfite conversion utilizes low and high annealing temperatures.