Fig 3.

Multilineage differentiation of the passaged-3 human mesenchymal stem cells. A-B) osteogenic differentiation culture was positively stained red with alizarin red. In this culture bone specific genes including osteocalcin and collagen I were expressed. C-D) Lipid droplets in adipogenic culture appeared red upon oil red staining. PPAR gamma and PPAR alpha were expressed in adipocytic cells. E-F) the sections from micro mass culture for cartilage differentiation were metachromatically stained purple with toloidine blue. Cartilage related genes including aggrecan and collagen II were expressed at chondrogenic culture (Bar= 500 µm)