Table 1. Primers used in RT-PCR.

Genes	Primer sequences (5'-3')	Annealing temperature (℃)	Length bp	Gene bank code
beta actin	F:5'tcc ctg gag aag agc tac g3'	60	131	NM-00110103
	R:5'gta gtt tcg tgg atg cca ca 3'
Osteocalcin	F:5'ggcagcgaggtagtgaagag3'	56	196	NM-199173.3
	R:5'cagcagagccacaccctagac3'
COL IA	F:5'gtg gtg aca agg gtg aga cac3'	62	225	NM-000088
	R:5'caa cag gac cag cat cac cag3'
PPARα	F:5'tgc tat cat ttg ctg tgg ag3'	60	175	NC_000022.10
	R:5'act ccg tct tct tga tga t3'
PPARॕ	F:5'cta aag agc ctg cga aag 3'	60	186	NC_000003.11
	R:5'tgt ctg tct ccg tct tct tg3'
COL2A	F:5'tct acc cca atc cag caa ac 3'	60	170	NC_000012.11
	R:5'gcg tag gaaggt cat ctg ga3'
Aggrecan	F:5'ctg gac aag tgc tat gcc g 3'	58	191	NC_000015.9
	R:5'gaa gga acc gct gaa atg c3'