FIG. 4.

LPS/GalN-induced liver apoptosis in TMX^−/− mice. (A, B) Apoptotic cells were detected by TUNEL assays performed on liver sections prepared from LPS/GalN-treated wild-type (+/+) and TMX^−/− mice. Bars 100 μm. (C, D) Liver extracts were prepared from mice 24 h post-LPS/GalN administration and analyzed by immunoblotting with the indicated antibodies. The full-length (asterisk) and the cleaved fragments of PARP (arrowhead) are indicated (C). The relative expression levels of Bax and FLIP were quantified and normalized to TUB (D). Bax, Bcl2-associated X protein; FLIP, FLICE inhibitory protein; PARP, poly(ADP–ribose) polymerase; TUNEL, terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling.