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TUNEL assay. To evaluate the effect of 5-FU on the HLF, HLF-R4, and HLF-R10 cells, a TUNEL assay was used to confirm the apoptotic changes. TUNEL staining shows a dramatic increase in the number of cells that were stained green, which indicates that apoptosis occurred in HLF cells exposed to 5-FU. 5-FU-induced apoptosis is inhibited in the HLF-R4 and HLF-R10 cells. The experiments were repeated three times with similar results. Scale bar = 50 μm.
