Figure 4.
Pp-FHY1 Is Essential for FR Light–Induced Gene Expression.
(A) Protonemata cultures of P. patens wild type (WT) and Pp-fhy1 mutant lines were dark adapted and exposed to either R light (28 μmol m−2 s−1) or FR light (16 μmol m−2 s−1). Samples for quantitative RT-PCR analyses were harvested after 1, 3, and 6 h of light treatment or darkness. The expression levels of FNR, ASN, and COL2 were normalized to the levels of 26S rRNA. Expression levels in darkness were set to 1. Error bars represent se of technical replicates, n = 3. An independent biological replicate is shown in Supplemental Figure 7 online.
(B) RT-PCR analysis of Pp-fhy1 mutants. Pp-fhy1 knockout lines were generated using gene targeting. Two independent Pp-fhy1 mutant lines were used for RT-PCR analysis with primers specific for either Pp-FHY1 or Pp-EF1α.