Figure 6.
Pp-FHY1 Is Essential for HIR-Like Responses to High Fluence Rate FR Light.
(A) Spore germination in FR light depends on Pp-FHY1. Spores from wild-type (WT) P. patens plants and two independent Pp-fhy1 mutant lines were irradiated for 8 d with continuous FR light (18 μmol m−2 s−1). To ensure that Pp-fhy1 mutant spores irradiated with FR light were viable, they were irradiated for an additional 5 d with W light after the FR light treatment. The bar plot shows significantly reduced germination rate in Pp-fhy1 mutants (Fisher’s exact test P < 3.4e-05). Bar = 100 μm.
(B) Pp-FHY1 is essential for protonemata growth in FR light. Wild-type and Pp-fhy1 mutant P. patens cultures were grown for 13 d in FR light (18 μmol m−2 s−1). Bar = 100 μm.
(C) FR light–induced gametophore growth requires Pp-FHY1. Wild-type and Pp-fhy1 mutant P. patens gametophores were grown for 11 d in FR (18 μmol m−2 s−1). Bar = 500 μm.
[See online article for color version of this figure.]