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. 2013 Jan 4;25(1):215–228. doi: 10.1105/tpc.112.106377

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© 2013 American Society of Plant Biologists. All rights reserved.

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Figure 1. — Light-Dependent Transcription and Translation of dsCYC2.

(A) Schematic representation of the HA marker construct.

(B) Transcript levels of dsCYC2 (solid line) and HA-tagged dsCYC2 (dashed line) during a 60-min time course after illumination (A.I.) of 24-h dark-adapted HA marker cells. Values were normalized against those obtained for histone H4 and then rescaled to the gene expression levels at 0 min after illumination (=1). Error bars represent se of three technical replicates.

(C) dsCYC2-HA protein levels during a 12-h (top panel) and 60-min (bottom panel) time course after illumination of 24-h dark-adapted HA marker cells. −, Negative control (wild-type 4 h light); +, positive control (HA 4 h light). LC, loading control by Coomassie blue staining.