Figure 6.

Posttranslational Regulation of dsCYC2 Induction upon Light Exposure.

(A) Wild-type (WT) cultures were synchronized by 24-h dark treatment (Dark) and then exposed to light for 0.5 (30 minL), 1 (1 hL), or 3 h (3 hL) in the absence (dark gray) or presence (light gray) of 2 μg/mL CHX. Relative expression levels of dsCYC2 are shown. Values were normalized against H4 expression levels and then rescaled to the gene expression levels of the dark sample (=1). Error bars represent se of two independent experiments.

(B) AUREO1a protein levels during a 60-min time course after illumination (A.I.) of 24-h dark-adapted HA marker cells. LC, loading control by Coomassie blue staining.