Figure 7.

Activation of the dsCYC2 Promoter by AUREO1a and bZIP10.

(A) Y2H protein–protein interaction assay. Yeast cells were cotransformed with bait and prey plasmid as indicated. Cotransformation was analyzed on medium lacking Leu and Trp (+His). Cotransformants were tested for their ability to activate the His marker gene by assessing yeast growth on medium lacking Leu, Trp, and His (-His). Constructs containing GUS were used as negative controls. For each combination, three independent colonies were screened, one of which is shown.

(B) Protoplast transactivation assay using pdsCYC2:fLUC as reporter, p35S:rLUC as normalization, and p35S:AUREO1a and p35S:bZIP10 as effector constructs. Luciferase activity of the control was arbitrarily set to 1. Error bars represent se of three biological replicates (*P ≤ 0.05, two-sided t test).