Effects of Constitutive and Inducible Overexpression of FCP1.
(A) and (B) Shoot apex (A) and in situ hybridization showing spatial expression pattern of OSH1
(B) in a wild-type seedling grown in soil 30 d after germination.
(C) and (D) Generating shoot (C) with pACT1-FCP1 and its shoot apex (D). Arrowhead indicates a terminated meristem (D).
(E) and (F) Transgenic shoot with pACT1-GVG>FCP1 (without DEX).
(G) and (H) Transgenic shoot with pACT1-GVG>FCP1 (10 µM DEX). Arrowhead indicates an abnormal meristem.
(I) and (J) Spatial expression pattern of OSH1 in the shoot apex of a transgenic shoot with pACT1-GVG>FCP1. Mock (I); 50 µM DEX
(J). Arrowhead indicates an abnormal meristem.
(K) and (L) Spatial expression pattern of FON2 in the shoot apex of a transgenic shoot with pACT1-GVG>FCP1. Mock (K); 50 µM DEX
(L). Seedlings were grown for 7 d from germination in the presence or absence of DEX ([E] to [L]).
alp, abnormal leaf primordia; mlp, malformed leaf primordia; P1-P5, plastochrons 1 to 5; WT, the wild type. Bars = 50 µm in (A), (B), (D), (F), and (H) to (L), 1 mm in (C), and 1 cm in (E) and (G).