Figure 7.

WOX4 Overexpression and Cytokinin Action.

(A) Phenotypes of calli constitutively overexpressing WOX4.

(B) Phenotypes of calli and regenerating shoots grown on different concentrations of BAP. NAA, naphthalene acetic acid.

(C) Content of iP and cZ in calli with empty vector (blue) or with pACT1-WOX4 (red). tZ was undetectable in either type of calli. Error bars indicate se; n = 6.

(D) Shoot formation from calli overexpressing WOX4 in a hormone-free medium.

(E) Effect of FCP1 peptide on calli overexpressing WOX4. Calli with abnormal shoot-like structures (as in [A]) were grown in regeneration liquid medium in the absence or presence of FCP1 peptide (20 µM) for 7 d with shaking (left). Shoot regeneration (shown on the right) was observed in the presence of FCP1 peptide, whereas no change was observed in the absence of FCP1.

Bars = 5 mm in (A), (B), and (E).