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HY5 Has Transcriptional Repression Activity.
(A) Diagram of various constructs used in this assay.
(B) Plasmid combinations of LUC reporter, GUS internal control, and effectors were cotransformed into Arabidopsis protoplasts. The protoplasts were incubated in weak light for 16 h, and relative activity was expressed as the ratio of LUC to GUS activity. Data represent the mean ± sd of three biological replicates.
