Table 3. Sugar Composition Analysis of Sequential Solvent Extracts of AIRs (Cell Walls) from apap1-3 Aerial Tissues versus Wild-Type Aerial Tissues.
| Sample |
Ara | Xyl | Fuc | Rha | Glc | Gal | Man | GlcA | GalA | ||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| μg/mg of Each Sample | |||||||||||||||||
| Oxalate | apap1-3 | 61.6 ± 12.6 | 10.3 ± 0.3* | 5.6 ± 0.3 | 67.3 ± 5.6* | 12.0 ± 5.6 | 60.4 ± 14.2 | 6.8 ± 2.5 | 59.4 ± 14.3 | 450.8 ± 79.4* | |||||||
| Wild type | 33.7 ± 20.0 | 7.6 ± 0.8* | 4.8 ± 1.8 | 28.9 ± 4.8* | 6.2 ± 0.1 | 36.1 ± 17.7 | 2.5 ± 0.2 | 94.8 ± 19.2 | 145.1 ± 29.4* | ||||||||
| Carbonate | apap1-3 | 94.0 ± 21.6 | 49.1 ± 21.4 | 5.4 ± 2.3 | 62.9 ± 6.5 | 55.6 ± 39.2 | 62.6 ± 12.4 | 8.0 ± 2.3 | 30.0 ± 11.3 | 181.2 ± 12.9* | |||||||
| Wild type | 43.1 ± 22.4 | 12.6 ± 7.8 | 2.5 ± 0.4 | 39.3 ± 13.3 | 11.7 ± 0.7 | 45.2 ± 17.3 | 5.5 ± 3.7 | 34.5 ± 14.7 | 57.9 ± 36.3* | ||||||||
| 1 M KOH | apap1-3 | 23.9 ± 11.1 | 218.0 ± 16.5 | 0.7 ± 0.4 | 15.3 ± 3.0 | 61.6 ± 24.2 | 13.4 ± 3.3 | 3.8 ± 1.3 | 11.8 ± 5.5 | 23.4 ± 6.1 | |||||||
| Wild type | 22.6 ± 11.9 | 239.2 ± 72.9 | 1.2 ± 1.2 | 7.7 ± 1.1 | 110.3 ± 69.3 | 19.2 ± 7.6 | 2.0 ± 0.1 | 19.0 ± 0.4 | 23.0 ± 8.9 | ||||||||
| 4 M KOH | apap1-3 | 29.8 ± 6.0 | 364.4 ± 99.6 | 10.4 ± 3.2 | 20.3 ± 4.4 | 85.0 ± 22.7 | 42.2 ± 11.5 | 17.4 ± 4.3 | 25.2 ± 6.1 | 41.4 ± 14.0 | |||||||
| Wild type | 17.5 ± 11.3 | 201.7 ± 48.5 | 6.0 ± 2.8 | 12.7 ± 5.5 | 60.3 ± 17.3 | 36.5 ± 18.5 | 22.8 ± 20.3 | 16.0 ± 5.5 | 36.5 ± 14.9 | ||||||||
| Chlorite | apap1-3 | 92.4 ± 21.5 | 22.0 ± 6.5 | 5.0 ± 3.4 | 43.0 ± 14.7 | 164.1 ± 48.7 | 39.1 ± 4.9 | 3.7 ± 0.8 | 22.8 ± 13.9 | 74.0 ± 23.1 | |||||||
| Wild type | 53.5 | 5.2 | 5.2 | 25.6 | 35.0 | 45.3 | 1.3 | 7.7 | 62.6 | ||||||||
| PC 4 M KOH | apap1-3 | 68.0 ± 20.0 | 388.9 ± 82.0 | 9.9 ± 2.1 | 49.4 ± 17.4 | 83.1 ± 20.0 | 40.5 ± 5.8 | 12.8 ± 2.4 | 19.5 ± 11.9 | 96.7 ± 11.4 | |||||||
| Wild type | 18.1 ± 15.0 | 91.5 ± 46.1 | 2.5 ± 2.5 | 14.9 ± 9.5 | 25.0 ± 12.2 | 17.4 ± 11.7 | 10.8 ± 8.9 | 14.2 ± 0.2 | 37.2 ± 18.9 | ||||||||
Three biological samples (plants) of mutant and two wild-type biological samples (plants) were analyzed and the data were averaged, respectively. Data are μg/mg of each extract. The same extracts were also analyzed in the glycome profiling analyses shown in Figure 5 and Supplemental Figure 7 online. The differences between bold mutant and wild type numbers for each solvent set are statistically significant (two-tailed t test, *P value < 0.05). The numbers are presented as mean ± se. PC, postchlorite.