Figure 3.

LR Growth Is Hypersensitive to ABA Treatment and Mutants Disrupting the ABA Signaling Pathway Affect Salt-Mediated Suppression of LR Growth.

(A) and (B) Relative PR and LR length quantified 4 dpt to media supplemented with 1 or 10 μM ABA (A) or 1 or 10 μM (B) ACC (n > 20 seedlings).

(C) Mutants disrupting ABA biosynthesis, signal transduction, or transcriptional regulation were surveyed for defects in the response to 100 mM NaCl. The average length of all LRs per seedling was measured and the percentage of difference between salt stress and standard conditions was calculated and shown in the graph. Mutations in the Ler background are shown as white bars, Ws background as light-gray bars, and Col-0 background as dark-gray bars (n > 15 seedlings). TF, transcription factor.

All seedlings grown for 6 dpg and average LR length per seedling measured 4 dpt. Error bars indicate se. Asterisks mark significant changes in salt response based on a Student’s t test, P value < 0.05, with Bonferroni correction.

[See online article for color version of this figure.]