Figure 5.

Progression of Complex PD Formation in Pavement Epidermal Cells.

(A) The first complex PDs appear at the three-way junctions between epidermal cells (arrowheads). Bar = 20 μm for (A) to (C).

(B) Subsequent PDs appear at the lobed tips of pavement epidermal cells (arrowheads).

(C) With continued development of the epidermis, complex PDs appear in the intervening wall regions between lobes (general PD labeling).

(D) Skeleton cell profiles, generated using ImageJ, are used to determine the positions of lobes.

(E) Quantitative assessment of the appearance of complex PDs at three-way junctions, lobe tips, and general regions, respectively, as a function of increasing complex PD numbers in a sample of leaves.