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Expression-based functional constraint (eFC)
(A) Genes expressed in more organs are more conserved in protein sequence than those expressed in fewer organs.
(B) to (D) Protein evolutionary rates are negatively correlated with eFCs. The evolutionary rates are computed as the Poisson-corrected protein distance between the orthologs in any pair of Arabidopsis, rice, and maize.
[See online article for color version of this figure.]
