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. 2011 Dec 15;40(4):1058–1065. doi: 10.3892/ijo.2011.1299

Figure 2.

Figure 2

Pretreatment with gomisin N promotes TRAIL-mediated apoptosis. (A) Hela cells were pretreated with 100 μM gomisin N for 30 min and then treated with TRAIL (100 ng/ml) for the indicated hours. Cell lysates were collected and subjected to Western blot analysis to detect the cleavage of PARP-1. (B) Gomisin N morphologically enhanced the cell death induced by TRAIL in HeLa cells. Left, cells treated with TRAIL for the indicated hours; right, cells pretreated with gomisin N for 30 min, followed by TRAIL for the indicated hours. Representative images of cells were photographed using a normal light microscope. Magnification, ×100. (C) Cells were treated with gomisin N for 30 min, followed by TRAIL for 6 h. Cells were stained with Annexin V-FITC and analyzed by FACS.