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. 2011 Dec 15;40(4):1058–1065. doi: 10.3892/ijo.2011.1299

Figure 3.

Figure 3

Combined treatment with gomisin N and TRAIL induces typical caspase-dependent apoptosis. (A) HeLa cells were pretreated with 10 μM z-VAD-FMK (z-VAD) for 30 min, followed by combined treatment with gomisin N (100 μM) for 30 min and then TRAIL (100 ng/ml) for another 3 h. Cells were collected to detect the cleavage of caspase-3, caspase-8 and PARP-1 by Western blot analysis. (B) HeLa cells were pretreated with z-VAD-FMK and gomisin N in a similar way and then treated with TRAIL for another 24 h. Cell viability was determined by WST-1 assay. Data are presented as the mean ± SD of six independent experiments; **P<0.01 vs. control group; ##P<0.01 vs. combined treatment group with gomisin N and TRAIL. (C) HeLa cells were pretreated with z-VAD-FMK and gomisin N in a similar way and then treated with TRAIL for another 3 h. Cells were stained with Annexin V-FITC and analyzed by FACS.