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Effect of gomisin N on TRAIL decoy receptors and various apoptosis regulatory proteins. (A) HeLa cells were treated with gomisin N (100 μM) for the indicated hours. The mRNA levels of DcR1 and DcR2 were measured by real-time RT-PCR. (B) HeLa cells were pretreated with gomisin N for 30 min, followed by treatment with TRAIL (100 ng/ml) for 3 h. Cell lysates were collected and subjected to Western blot analysis to detect apoptosis regulatory proteins.
