Figure 3.

Effects of PLEO on phosphorylation of ERK-1/2 and JNK-1/2 in YD-8 cells. (A) YD-8 cells were treated without or with PLEO (60 μg/ml) for the indicated times. At each time, whole cell lysates were prepared and analyzed by Western blotting. The image is a representative of three independent experiments. (B) The same immunoblot as (A) was stripped and then reprobed with an antibody which recognizes total expression levels of ERK-1/2 or JNK-1/2. The image is a representative of three independent experiments. p-ERK-1/2, phosphorylated ERK-1/2; T-ERK-1/2, total ERK-1/2; p-JNK-1/2, phosphorylated JNK-1/2; T-JNK-1/2, total JNK-1/2.