Figure 4.

Effect of PLEO on generation of intracellular ROS in YD-8 cells. YD-8 cells were primarily loaded with DCFH-DA (20 μM) for 20 min and then treated without or with PLEO (60 μg/ml) for 10 min (A), 30 min (B) or 2 h (C). At each time, cells were harvested, washed twice with PBS and suspended in PBS. The ROS generation was measured by the DCF fluorescence intensity (FL-1, 530 nm) from 10,000 cells with a FACS Calibur flow cytometer (Becton-Dickinson). Data analysis was carried out using CellQuest program. The fluorescence is expressed as a histogram. Each picture in (A), (B) or (C) is a representative of three independent experiments.