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TGF-β1 activates ERK/MAPK and PI3K pathways in RMG-I-H cells. (A) Western blot analysis was performed to detect the expression of p-ERK, ERK, p-Akt and Akt in RMG-I-H cells at different time-points after TGF-β1 stimulation. (B) Relative intensity of p-ERK, ERK, p-Akt and Akt protein were expressed as means in bar graphs, significant differences from control cells were noted as *p<0.01.
