Skip to main content
. 2011 Dec 20;40(4):1210–1219. doi: 10.3892/ijo.2011.1310

Figure 5.

Figure 5

Inhibition of SVB-induced apoptosis by caspase-3 inhibitor in A549 cells. (A) A549 cells were treated with z-DEVD-fmk (50 μM) for 1 h before challenge with 5 μg/ml of SVB for 24 h. Cells were stained with DAPI for 10 min and photographed with a fluorescence microscope using a blue filter (magnification ×400). (B) Cell proliferation was determined using the MTT assay after 24 h in the presence of the caspase-3 inhibitor z-DEVD-fmk (50 μM) for 1 h before SVB (5 μg/ml) treatment. Data are expressed as mean ± SD of three independent experiments. (C) Cells grown under the same conditions as (A) were evaluated for sub-G1 DNA content using a flow cytometer. Data are reported as mean ± SD of three independent experiments. The significance was determined by Student’s t-test (*p<0.05 vs. untreated control).