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. 2011 Dec 20;40(4):1071–1078. doi: 10.3892/ijo.2011.1313

Figure 4.

Figure 4

DDTC induces caspase-3-dependent apoptosis in HHV-8-infected cells. (A) HHV-8-infected cells (BCBL-1, BC-1 and BC-3) were treated with DDTC at the indicated dose and incubated at 37°C for 24 h. After incubation, cells were recovered and subjected to MTT assay. To confirm the specificity of DDTC treatment for HHV-8 infected cells, we tested the effect of DDTC on chronic myelogenous leukemia cell line K562 (HHV-8-uninfected cells). (B) Apoptosis was evaluated after treating HHV-8-infected and HHV-8-uninfected cells with 3 μM DDTC, and staining with Annexin V at 48 h. Flow cytometry profile represents Annexin V-FITC staining in x axis and PI in y axis. The number represents the percentage of early apoptotic cells (lower right quadrant) and late apoptotic cells (upper right quadrant) in each condition.